required reading:
Hammer, Menzel (1998) Multiple Sites of Associative Odor Learning as Revealed by Local Brain Microinjections of Octopamine in Honeybees. Learn&Mem 5:146–156.
pdf-file
required reading:
Hammer, Menzel (1998) Multiple Sites
of Associative Odor Learning as Revealed by Local Brain Microinjections of Octopamine
in Honeybees. Learn&Mem 5:146–156.
pdf-file
questions:
1.
What is known about the VUMmx1 that is pertinent to this study?
2. Describe the behavioral paradigm. Why was octopamine (OA) the neurotransmitter
of choice? How does the training protocol in this study compare with the training
protocols in other classical conditioning paradigms or other olfactory learning
studies (Aplysia, Pavlov's dogs, etc.)?
3. What is the proposed control for the potential general excitability change
with OA injection? Do you buy it?
4. The authors claim that the pairing of OA in the AL with odor supports acquisition
but not consolidation. What data supports this claim? What is their claim for
the role of the MB in classical conditioning?
5. The authors suggest that ablation of specific brain structures would be useful
in studying their roles in learning and memory. Based on the evidence presented
what would you predict would be the behavioral result in ablation of the AL,
MB and LPL?
6. What would you predict behaviorally if the odor stimulus (CS)was presented
24 hours after training with paired OA injections into the AL versus paired
OA injections into the MB?
7. Propose a model for how associative learning occurs in the honeybee.
PDF-file
of power point lecture (about 1 MB)
background reading:
Ito, Suzuki, Estes, Ramaswami,
Yamamoto, Strausfeld (1998).
The Organization of Extrinsic Neurons and Their Implications in the Functional
Roles of the Mushroom Bodies in Drosophila melanogaster Meigen. Learn&Mem
5:52–77. pdf-file
Rybak,
Menzel (1998) Integrative Properties of the Pe1 Neuron, a Unique Mushroom Body
Output Neuron. Learn&Mem 5:133–145. pdf-file
Pascual, Preat (2001) Localization of Long-Term Memory Within the Drosophila
Mushroom Body. Science 294:1115-1117. pdf-file
Dubnau,
Grady, Kitamoto, Tully (2001) Disruption
of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition
of memory. Nature 411:476-480. pdf-file
Strausfeld (2002) Organization of the Honey Bee Mushroom Body: Representation
of the Calyx Within the Vertical and Gamma Lobes. J comp Neurol 450:4–33. pdf-file
Links:
flybrain
Interested in 3D reconstruction:
Amira (gallery)
OpenDX
