Figure 7 - Enhancer-trap Patterns that Demonstrate the Subdivision of the R4-type eb

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Figure 7 - Enhancer-trap patterns that demonstrate the subdivision of the R4-type eb category into R4m and R4d types.

This frontal perspective represents a confocal reconstruction of ~ 50 µm. Some c42 axons (green) enter the neuropil at the perimeter and arborize in a distal zone consistent with the R4 designation. However, in eight specimens examined, no more than four c42 cell bodies (green) were double labeled by another R4 enhancer pattern, pWF22-6 (red), indicating that they represent distinct R4 types. R4d axons arborize in the most distal eb zone, while R4m occupy a more medial zone. Fan-shaped neuron cell bodies (asterisk) which project to the fb are also labeled by c42. The scale bar = 20 µm.

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