Figure 6 - Expression of EN and BSH in Wild Type and Mutant Brains
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Reconstruction of optical sections. Brain is visualized with anti-HRP antibody (green). Bar, 20 µm. (A, B, C) Expression of EN and EXD in wild type embryos at stage 14. Lateral images. Arrow, en-b1; arrowhead, en-b2; open arrow, en-b3. (A) Expression of EN (red/orange; Patel et al., 1989). The three groups of EN expressing cells in the brain appear as cell clusters. (B) Expression of EXD (blue). Bracket, strong EXD expression in a dorsal part of neuromere b1. Star, optic lobe primordia. (C) Merge of the images of A and B. Note the coexpression (pink) of EN and EXD in the en cells except for en-b1. (D) Expression of EN (red/orange) in wild type embryo at stage 16. (E, F). Expression of the EN protein (red/orange) in maternal and zygotic exd null embryos. (E) Stage 14. (F) Stage 16. Arrow, en-b1; arrowhead, en-b2; open arrow, en-b3. Mn, mandibular neuromere; Mx, maxillary neuromere; Lb, labial neuromere. Stars, epidermal EN clusters, which also are ectopically induced in exd mutant. Arrows in (F) indicate en-b1 and ectopic EN cells. (G-I) Expression of BSH (orange/red; Jones and McGinnis, 1993) in wild type (G) and mutants (H, I). Lateral views of stage 16 embryos. (H) Maternal and zygotic exd null mutant. (I) hth mutant. Arrows, BSH cell clusters in neuromere b1; arrowhead, ectopic BSH cells in more posterior regions.
|Flybrain Development Patterning Defects Figure 6|
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