Figure 3 - Axonal Tract Defects in exd and hth Mutant Brains
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Laser confocal microscopy of wild type and mutant embryos double labeled with anti-Fas II antibody (red/yellow) and neuron-specific anti-HRP antibody (green). Reconstruction of optical sections. (A, D, G, J) Wild type embryos, (B, E, H, K) Maternal and zygotic exd null embryos, (C, F, I, L) hth mutant embryos. (A-C) stage 12/3, (D-F) stage 14, (G-L) stage 16. (A-I) Lateral views, (J-L) dorsal views. Scale bar, 20 µm. Arrows in H and I indicate the clumped neural fibers. Brackets in K and L indicate the abnormal brain commissure. bc, brain commissure; bn, Bolwig's nerve; bo, Bolwig's organs; fc, frontal commissure; hy, hypocerebral ganglion; ol, optic lobe; pv, proventricule; rn, recurrent nerve. D/T, P1, P2l, P2m, P3l, P3m, P4l, and P4m indicate fiber tract founder clusters (Nassif et al., 1998).
|Flybrain Development Patterning Defects Figure 3|
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